Native cytokines do not bind to uromodulin (Tamm‐Horsfall glycoprotein)

FEBS Letters

Wingfield, Paul¹  Moonen, Peter¹  Gaffner, Raymond¹ 

[1] Glaxo Institute for Molecular Biology SA, PO Box, CH-1211 Geneva 24, Switzerland

关键词: Uromodulin;    Tamm-Horsfall glycoprotein;    Interleukin-1;    Tumour necrosis factor;    TH-glycoprotein;    Tamm-Horsfall glycoprotein;    TNF;    tumour necrosis factor;    IL-1;    interleukin-1;    GM-CSF;    granulocyte-macrophage colony stimulating factor;    HBcAg;    hepatitis core antigen;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    Con A;    concanavalin A;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(88)81446-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Uromodulin bound with high affinity to human tumour necrosis factor (TNF) coated on microtitre plates. This interaction was not competitively inhibited by native TNF in solution. No interaction was observed between immobilized uromodulin and TNF in the liquid phase unless conditions were chosen which denatured the latter protein. Recombinant interleukin-1α adsorbed on microtitre plates also interacted with uromodulin. However, gel filtration experiments demonstrated no interaction between the proteins in the liquid phase. These and additional results indicate that uromodulin interacts with denatured cytokines, but not with native, soluble cytokines.

【 授权许可】

Unknown