A ribosome‐associated inhibitor of in vitro nonsense suppression in [psi −] strains of yeast

FEBS Letters

Cox, Brain S.¹  McLaughlin, Calvin S.²  Tuite, Mick F.³ 

[1] Department of Plant Sciences, University of Oxford, Oxford OX1 3RA, England;Department of Biological Chemistry, University of California, Irvine, CA 92717, USA;Biological Laboratory, University of Kent, Canterbury CT2 7NJ, England

关键词: Nonsense suppression;    [psi] factor;    Ribosome;    Translation termination;    (Saccharomyces cerevisiae);   

DOI  :  10.1016/0014-5793(87)81158-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

All classes of tRNA-mediated nonsense suppression are much more efficient in yeast cell-free lysates prepared from a [psi ⁺] strain than in those prepared from an isogenic [psi ⁻] strain. Mixed [psi ⁺]/[psi ⁻] lysates do not support efficient suppression. Fractionation of the [psi ⁻] lysate demonstrated the presence of an inhibitor of in vitro suppression that is loosely associated with the 80 S ribosome. The data indicate that the inhibitor is a factor involved in the termination of translation in this simple eukaryote.

【 授权许可】

Unknown