Identification of the lesion in the stimulatory GTP‐binding protein of the uncoupled S49 lymphoma

FEBS Letters

Harris, Bruce A.¹  Rall, Thomas¹ 

[1] Merrell Dow Research Institute, 16, rue d'Ankara, 67084 Strasbourg, France

关键词: G-protein;    Adenylate cyclase;    Uncoupled mutant;    (S49 lymphoma);   

DOI  :  10.1016/0014-5793(87)80486-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The stimulatory GTP-binding protein (G_s) of the uncoupled mutant of S49 lymphoma cells is deficient in its ability to transduce hormonal signals from ligand-bound β-adrenergic receptors to the catalytic component of adenylate cyclase. In order to define the genetic defect in the G_s of uncoupled S49 cells, a complementary DNA clone encoding the α-subunit of G_s was analyzed and the deduced primary structure of the defective subunit compared to that of the wild-type subunit. A single nucleotide transversion was found that coded for a proline rather than an arginine at residue 389. The results indicate a domain of the α-subunit of G_s that specifically interacts with hormone receptors.

【 授权许可】

Unknown