Immunoaffinity‐purified opiate receptor specifically binds the δ‐class opiate receptor ligand, cis‐(+)‐3‐methylfentanylisothiocyanate, SUPERFIT

FEBS Letters

Rice, Kenner C.¹  Blalock, J.Edwin²  DeCosta, Brian¹  Bost, Kenneth L.²  Carr, Daniel J.J.²  Jacobson, Arthur E.¹ 

[1] Section on Drug Design and Synthesis, Laboratory of Neuroscience, NIDDK, NIH, Bethesda, MD 20892, USA;University of Alabama at Birmingham, Department of Physiology and Biophysics, University Station, Birmingham, AL 35294 USA

关键词: Complementary peptide;    cis-(+)-3-Methylfentanylisothiocyanate;    Opiate receptor;   

DOI  :  10.1016/0014-5793(87)80468-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using an antibody generated against the opiate receptor on NG108-15 cells, we recently purified the putative receptor from this hybrid cell line. We herein report that the purified receptor complex specifically binds tritiated cis-(+)-3-methylfentanylisothiocyanate (SUPERFIT), with the predominant binding associated with a 58 kDa polypeptide chain. Consistent with these findings is the in situ labeling of a 58 kDa protein with [³H]SUPERFIT on NG108-15 cells.

【 授权许可】

Unknown