Different abilities of two distinct Fcγ receptors on guinea pig polymorphonuclear leukocytes to trigger the arachidonic acid metabolic cascade

FEBS Letters

Koyama, Jiro¹  Nakamura, Tohoru¹  Sato, Masaki¹ 

[1] Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060, Japan

关键词: Arachidonic acid;    Fcγ receptor;    Anti-Fcγ receptor antibody;    Immune complex;    Polymorphonuclear leukocyte;    PMN;    guinea pig polymorphonuclear leukocytes;    FcγR;    Fcγ receptor;    Fcγ2R;    Fcγ receptor for IgG2;    Fcγ1/γ2R;    Fγ receptor for IgG1 and IgG2;    OA;    hen ovalbumin;    KRPG;    Krebs Ringer phosphate buffer supplemented with 5 mM D-glucose;    pH 7.4;    OA-IgG1;    ovalbumin-complexed IgG1 antibody;    OA-IgG2;    ovalbumin-complexed IgG2 antibody;   

DOI  :  10.1016/0014-5793(87)80416-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nature of signals transmitted by two Fcγ receptors (Fcγ₂R for IgG2 and Fcγ₁/γ₂R for both IgG2 and IgG1) on guinea pig polymorphonuclear leukocytes was investigated. The specific binding of hen ovalbumin (OA)-complexed IgG1 antibody to Fcγ₁/γ₂R did not seem to trigger the release of [³H]arachidonic acid from the cells prelabeled with [³H]arachidonic acid. In contrast, marked release occurred on the binding of OA-complexed IgG2 antibody to the cells. This reaction was not inhibited, but rather enhanced, by the Fab′ of a monoclonal antibody to Fcγ/γ₂R. Therefore, a signal for the activation of the arachidonic acid metabolic cascade was found to be transmitted by Fcγ₂R, but scarcely by Fcγ₁/γ₂R.

【 授权许可】

Unknown