Nerve Growth Factor Modulates Fc Gamma Receptor Expression on Murine Macrophage J774A.1 Cells

Journal of Veterinary Medical Science

Akane TANAKA²  Eiichi HONDA¹  Hiroshi MATSUDA²  Yasuhiko SUSAKI² 

[1] Deparment of Veterinary Microbiology, Faculty of Agriculture, Tokyo University of Agriculture and Technology;Department of Veterinary Clinic, Faculty of Agriculture, Tokyo University of Agriculture and Technology

关键词: Fcγ receptor;    macrophage;    nerve growth factor;    p140trk;   

DOI  :  10.1292/jvms.60.87

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(36)Cited-By(1)We assessed the effect of nerve growth factor (NGF) on Fc gamma receptor (FcγR) expression on murine monocyte-macrophage J774A.1 cells. Flow cytometric analysis showed that treatment with various doses of NGF led to a rapid increase of FcγRI and FcγRII/III expression in a dose-dependent manner. Northern blot analysis with digoxigenin-labeled oligonucleotide probes demonstrated that NGF induced a marked increase in mRNA synthesis of FcγRI and FcγRII, but not FcγRIII. Since pretreatment with K-252a, a tyrosine kinase inhibitor, inhibited the stimulatory effect of NGF completely, the FcγR expression augmented by NGF may be mediated through p140trk with a tyrosine kinase domain. These results suggest that NGF may be a novel cytokine that is able to increase FcγRI and FcγRII expression on macrophages.

【 授权许可】

Unknown