Sequential mechanism of refolding of carbonic anhydrase B

FEBS Letters

Semisotnov, Gennady V.²  Kutyshenko, Victor P.¹  Ptitsyn, Oleg B.²  Blanck, Jürgen³  Ebert, Bernd³  Rodionova, Natalya A.² 

[1] Institute of Biological Physics, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR;Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR;Central Institute of Molecular Biology, Academy of Sciences of the GDR, 1115 Berlin, GDR

关键词: Carbonic anhydrase B;    Refolding kinetics;    Kinetic intermediate;    Hydrophobic probe;    1H-NMR;    ESR;    Energy transfer;    Fluorescence;    t ½;    half-time of the process;    CD;    circular dichroism;    ANS;    8-anilino-1-naphthalenesulfonate;   

DOI  :  10.1016/0014-5793(87)80412-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The kinetics of refolding of bovine carbonic anhydrase B was studied by a variety of methods over a wide range of times (from milliseconds to hours). It has been shown that protein refolding proceeds through three stages. At the first stage (t _½≈0.03 s) hydrophobic clusters and a compact state of the chain are formed. At the second stage (t _½≈140 s) hydrophobic clusters are desolvated and the rigid native-like hydrophobic core is formed. At the third stage (t _½≈600 s) the native active protein is formed.

【 授权许可】

Unknown