期刊论文详细信息
FEBS Letters | |
Sequential mechanism of refolding of carbonic anhydrase B | |
Semisotnov, Gennady V.2  Kutyshenko, Victor P.1  Ptitsyn, Oleg B.2  Blanck, Jürgen3  Ebert, Bernd3  Rodionova, Natalya A.2  | |
[1] Institute of Biological Physics, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR;Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR;Central Institute of Molecular Biology, Academy of Sciences of the GDR, 1115 Berlin, GDR | |
关键词: Carbonic anhydrase B; Refolding kinetics; Kinetic intermediate; Hydrophobic probe; 1H-NMR; ESR; Energy transfer; Fluorescence; t ½; half-time of the process; CD; circular dichroism; ANS; 8-anilino-1-naphthalenesulfonate; | |
DOI : 10.1016/0014-5793(87)80412-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The kinetics of refolding of bovine carbonic anhydrase B was studied by a variety of methods over a wide range of times (from milliseconds to hours). It has been shown that protein refolding proceeds through three stages. At the first stage (t ½≈0.03 s) hydrophobic clusters and a compact state of the chain are formed. At the second stage (t ½≈140 s) hydrophobic clusters are desolvated and the rigid native-like hydrophobic core is formed. At the third stage (t ½≈600 s) the native active protein is formed.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020289890ZK.pdf | 228KB | download |