Progressin: A novel proliferation‐sensitive and cell cycle‐regulated human protein whose rate of synthesis increases at or near the G1/S transition border of the cell cycle

FEBS Letters

Celis, Ariana¹  Ratz, Gitte Petersen¹  Celis, Julio E.¹ 

[1] Department of Medical Biochemistry, Ole Worms Alle, Building 170, University Park, Aarhus University, DK-8000 Aarhus C, Denmark

关键词: Cell proliferation;    Cell cycle;    Initiation of DNA replication;    Transformation;    Cancer;    NEPHGE;    nonequilibrium pH gradient electrophoresis;    PCNA;    proliferating cell nuclear antigen;    2D;    two dimensional;   

DOI  :  10.1016/0014-5793(87)80296-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel proliferation-sensitive and cell cycle-specific basic protein, termed progressin (M _r,=33 000), has been identified in proliferating human cells of epithelial, fibroblast and lymphoid origin. Progressin is synthesized almost exclusively during the S-phase of transformed human amnion cells (AMA). Increased synthesis of this protein is first detected late in G₁, at or near the G₁/S transition border, reaches a maximum in mid to late S-phase, and declines thereafter. Contrary to histones, progressin synthesis is not coupled to DNA replication. As expected for an S-phase-specific protein, no detectable synthesis of progressin was observed in non-proliferating human MRC-5 fibroblasts and epidermal basal keratinocytes. Elevated, but variable levels of this protein were observed in proliferating normal fibroblasts and transformed cells of fibroblast, epithelial and lymphoid origin. Taken together the above observations suggest that progressin may be a component of the common pathway leading to DNA replication and cell division.

【 授权许可】

Unknown