Mechanically induced electrical responses in murine mammary epithelial cells in primary culture

FEBS Letters

Maeno, Takashi³  Furuya, Kishio¹  Oka, Takami²  Enomoto, Koh-ichi¹  Edwards, Charles¹ 

[1] Laboratory of Cell Biology and Genetics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA;Laboratory of Molecular and Cellular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA;Department of Physiology, Shimane Medical University, Izumo, Shimane 693, Japan

关键词: K+ channel;    Hyperpolarization;    Membrane potential;    (Mammary cell);   

DOI  :  10.1016/0014-5793(87)80514-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In mouse mammary epithelial cells in primary culture, mechanical stimulation of a cell induced in other cells within the same colony a short depolarization of less than 15 m V with a duration of 1–8 s and a subsequent, prominent hyperpolarization of 6 m V lasting 10–40 s. Epidermal growth factor induces a spontaneous hyperpolarizing response in cultured mammary cells, and in cells treated with EGF mechanical stimulation produced a greater hyperpolarization, while the amplitude of the depolarizing response was not affected. The amplitude of the mechanically induced hyperpolarization was markedly reduced by quinine and tetraethylammonium, blockers of the Ca²⁺-dependent K⁺ channel. The results suggest that the Ca²⁺-dependent K⁺ channel was involved in the hyperpolarization.

【 授权许可】

Unknown