Tissue‐specific binding of a nuclear factor to the insulin gene promoter

FEBS Letters

Steiner, Donald F.¹  Sample, Clare E.¹ 

[1] Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637 USA

关键词: DNA-protein interaction;    DNA binding;    Insulin gene;    Tissue specificity;    (Rat);   

DOI  :  10.1016/0014-5793(87)80396-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a DNA-binding gel mobility shift assay, a protein-DNA complex was detected using the rat II insulin promoter and a nuclear extract from rat insulinoma cells. This complex was detected using extracts from other insulin-producing cell lines but was not found using nuclear extracts from non-insulin-producing pancreatic cell lines or other cell types. Binding of the tissue-specific protein to the labelled promoter fragment was effectively competed by both rat and human insulin genes but not by the SV40 TATA box. This protein-DNA interaction may be involved in tissue-specific regulation of insulin gene expression.

【 授权许可】

Unknown