A RIPE3b1‐like factor binds to a novel site in the human insulin promoter in a redox‐dependent manner

FEBS Letters

Read, Martin L¹  Masson, Margaret R¹  Docherty, Kevin¹ 

[1] Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK

关键词: Insulin gene;    Rat insulin protomer element 3b1;    2-ME;    2-mercaptoethanol;    DM;    diamide;    DTT;    dithiothreitol;    EFD3;    endocrine factor D3;    EMSA;    electrophoretic mobility shift assay;    HLH;    helix-loop-helix;    IEF1;    insulin enhancer factor 1;    IUF1;    insulin upstream factor 1;    NEM;    N-ethylmaleimide;    RIPE3;    rat insulin promoter element;    USF;    upstream stimulating factor;   

DOI  :  10.1016/S0014-5793(97)01352-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the human insulin gene, a regulatory sequence upstream of the transcription start site at −229 to −258 (the E2 element) binds a ubiquitous factor USF. The present study led to the identification of a second factor, D0, that binds to an adjacent upstream site, the C2 element, that has previously not been described. The results demonstrate that D0 exhibits similar properties to RIPE3b1, a factor shown to be an important determinant of insulin gene β-cell-specific expression. Binding of D0 to the C2 element was abolished by the oxidising agent diamide, and the alkylating agent N-ethylmaleimide. The results indicate that expression of the insulin gene may be regulated by a redox-dependent pathway involving RIPE3b1 or a RIPE3b1-like factor.

【 授权许可】

Unknown