In vitro evidence that the coat protein is the programming factor in alfalfa mosaic virus‐induced RNA synthesis

FEBS Letters

Jaspars, E.M.J.¹  Houwing, Corrie J.¹ 

[1] Department of Biochemistry, State University, PO Box 9505, NL-2300 RA Leiden, The Netherlands

关键词: Alfalfa mosaic virus;    RNA-dependent RNA polymerase;    Coat protein regulation;    Protoplast infection;   

DOI  :  10.1016/0014-5793(87)80952-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Viral RNA-dependent RNA polymerase preparations were isolated from cowpea mesophyll protoplasts 26 h after inoculation with the complete genome (RNAs 1, 2 and 3) and the incomplete genome (RNAs 1 and 2) of alfalfa mosaic virus. Although in incompletely inoculated protoplasts the ratio minus-/plus-strand RNA synthesis is much higher than in completely inoculated ones [(1983) Virology 124, 75–85)], both enzyme preparations produced largely plus-strand RNA on their endogenous templates. When made template-dependent both were strongly inhibited by small amounts of coat protein. The experiments show that among the two proteins encoded by RNA 3 only the (parental) coat protein can be responsible for the programming of the viral RNA polymerase to plus-strand synthesis.

【 授权许可】

Unknown