Coat protein blocks the in vitro transcription of the virion RNAs of alfalfa mosaic virus

FEBS Letters

Jaspars, E.M.J.¹  Houwing, Corrie J.¹ 

[1] Department of Biochemistry, State University, PO Box 9505, NL-2300 RA Leiden, The Netherlands

关键词: Alfalfa mosaic virus;    RNA dependence;    RNA polymerase;    Coat protein regulation;    YSMV;    yellow spot mosaic virus;    TYMV;    turnip yellow mosaic virus;    BMV;    brome mosaic virus;    SSC;    0.15 M NaCl;    0.015 M Na citrate;   

DOI  :  10.1016/0014-5793(86)81128-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a method described by Miller and Hall [(1983) Virology 125, 236-241] for brome mosaic virus, we succeeded in obtaining preparations of an alfalfa mosaic virus specified RNA polymerase that is totally dependent on added RNA. The enzyme makes full-size transcripts on each of the four virion RNAs. Transcription is inhibited by small amounts of coat protein subunits. This is in accordance with a model proposed by Nassuth and Bol [(1983) Virology 124, 75-85] which says that in a late stage of the infection cycle the coat protein is a factor that regulates the minus-strand RNA synthesis in a negative sense.

【 授权许可】

Unknown