Variations with position of replication errors due to exonuclease warm‐up

FEBS Letters

Lecomte, Philippe J.¹  Ninio, Jacques¹ 

[1] Institut Jacques Monod, Tour 43, 2 Place Jussieu, 75251 Paris Cedex 05, France

关键词: DNA polymerase I;    Processivity: Mismatch error;    Proofreading;    Mnemonic enzyme;    (E. coli);   

DOI  :  10.1016/0014-5793(87)80924-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A.A mismatch errors occuring during poly(dA) replication with the Klenow fragment of E. coli DNA polymerase I have been qualified. The A/T ratio measured for chains extended by 1–25 nucleotides decreases by a factor of at least 15 from beginning to end. The deduced true error rate may decrease by a factor of 2.5 at each successive nucleotide addition. When ddATP is used instead of dATP, the ddA/T ratio indicates little variation of the misincorporation probability with position. Thus, the accuracy improvement in the first case is due to a warm-up of the proofreading function.

【 授权许可】

Unknown