Is there a root effect in Xenopus hemoglobin?

FEBS Letters

Giardina, Bruno²  Perutz, Max F.³  Bellelli, Andrea¹  Brunori, Maurizio¹  Condo, Saverio² 

[1] Department of Biochemical Sciences and CNR Center of Molecular Biology, University of Rome ‘La Sapienza’, P. le Aldo Moro 5, 00185 Roma, Italy;Department of Experimental Medicine and Biochemical Sciences, University of Rome ‘Tor Vergata’, via O. Raimondo, 00100 Roma, Italy;Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge, England

关键词: Hemoglobin;    Allosteric transition;    Bohr effect;    DPG;    2;    3-diphosphoglycerate;    IHP;    inositol hexakisphosphate;    Mb;    myoglobin;    Hb;    hemoglobin;    Bis-tris;    bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane;   

DOI  :  10.1016/0014-5793(87)80372-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The reaction of Xenopus hemoglobin with oxygen and carbon monoxide has been reinvestigated over the pH range 8.5–6.0, in the absence and presence of organic phosphates (2,3-diphosphoglycerate or inositol hexakisphosphate), to establish if the tetramer can be stabilized in a T-quaternary state by protons and polyphosphate; the equilibrium and kinetic data indicate that Xenopus hemoglobin does exhibit a Root effect. These new results are discussed with reference to those reported by Bridges et al. [(1985) Resp. Physiol. 61, 125-136] on Xenopus blood and, more generally, to the molecular definition and the structural basis of the Root effect as an extreme form of the Bohr effect.

【 授权许可】

Unknown