Bradykinin inhibits potassium (M) currents in N1E‐ 115 neuroblastoma cells Responses resemble those in NG108‐15 neuroblastoma x glioma hybrid cells

FEBS Letters

Brown, David A.¹  Higashida, Haruhiro¹ 

[1] Laboratory of Biochemical Genetics, National Heart, Lung & Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Bradykinin;    Methacholine;    Inositol trisphosphate;    Membrane current;    M-current;    Neuroblastoma;    (Mouse);   

DOI  :  10.1016/0014-5793(87)80835-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Application of bradykinin to voltage-clamped N1E-115 mouse neuroblastoma cells evoked sequential outward and inward membrane currents, accompanied by an increase and decrease of membrane conductance, respectively. Methacholine produced an inward current with a decreased conductance. The outward current response to bradykinin was imitated by intracellular inositol 1,4,5-trisphosphate (IP₃). Bath application of phorbol dibutyrate induced an inward current and potentiated the response to IP₃. We conclude that the response of these cells to bradykinin is identical to that of NG108-15 hybrid cells, and therefore may be attributed to the dual effects of inositol trisphosphate and diacylglycerol formed by hydrolysis of phosphatidylinositide.

【 授权许可】

Unknown