Effect of unbalanced diets on incorporation of δ‐aminolevulinic acid into cytochrome P‐450

FEBS Letters

Oesch, F.³  Periquet, A.⁴  Robertson, L.W.²  Borin, C.⁴  Narbonne, J.F.¹  Amelizad, Z.³ 

[1] Laboratoire de Toxicologie Alimentaire, Université de Bordeaux I, F-33405 Talence, Bordeaux France;Graduate Center for Toxicology, 204 Funkhouser Bldg, University of Kentucky, Lexington, KY 40506-0054, USA;Institute for Toxicology, University of Mainz, D-6500 Mainz, FRG;Institut de Physiologie, Université de Toulouse III, F-31400 Toulouse, France

关键词: Cytochrome P-450 synthesis;    Dietary protein;    Polychlorobiphenyl;    Nutritional effect;    δ-Aminolevulinic acid;   

DOI  :  10.1016/0014-5793(87)80911-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The in vivo syntheses of two liver microsomal cytochromes P-450 PB_3a, P-450 UT₅₀ [(1987) Eur. J. Biochem., submitted] (M _r 50 000, 52 000) have been estimated by measuring the specific activity 2 h after i.p. administration of δ-[³H]aminolevulinic acid to male Sprague Dawley rats. The animals were fed either a standard rat chow (5% lard, 22% casein) or unbalanced diets (high lipid, 30% lard or low protein, 6% casein) with or without 50 ppm Phenoclor DP6. The high-lipid diet supported a more rapid body weight gain but had little impact on cytochrome P-450 content, expressed either per whole liver or per mg microsomal protein, and on the incorporation of the precursor into cytochrome P-450. The latter was determined by measuring the radioactivity incorporated into the cytochrome P-450 fraction, partially purified by afffinity chromatography, as well as into two cytochrome P-450 isozymes (M _r 50 000 or 52 000) purified by DEAE-52 cellulose ion-exchange chromatography. The low-protein diet, on the other hand, severely depressed body weight gain and cytochrome P-450 content as well as incorporation of radioactivity, the lower-M _r cytochrome (M _r 50 000) being particularly affected. However, when a potent inducer, Phenoclor DP6, was added to the low-protein diet, cytochrome synthesis was restored indicating that the effect was reversible.

【 授权许可】

Unknown