Triiodothyronine stimulates proliferation of osteoblast‐like cells in serum‐free culture

FEBS Letters

Froesch, E.R.¹  Ernst, M.¹ 

[1] Metabolic Unit, Dept of Medicine, University Hospital, CH-8091 Zürich, Switzerland

关键词: Serum-free culture;    Collagen synthesis;    Alkaline phosphatase;    Triiodothyronine;    (Osteoblast-like cell);   

DOI  :  10.1016/0014-5793(87)80896-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Primary rat ostcoblast-like cells (Ob) were grown under strictly serum-free conditions for up to 20 days. In the presence of triiodothyronine (T₃) at concentrations of 0.01 and 0.1 nM, Ob proliferation was enhanced. Moreover, a decrease of alkaline phosphatase (AP) activity, a differentiation marker for Ob, was prevented, whereas protein synthesis (collagen and noncollagen protein) was decreased. T₃ at much higher concentration (10 nM) had no significant effect on cell proliferation and matrix formation but decreased AP activity disproportionately. Thus, T₃ at close to physiological concentrations stimulates growth and maintains differentiation of Ob.

【 授权许可】

Unknown