Binding of low‐density lipoprotein to monolayer cultures of rat hepatocytes is increased by insulin and decreased by dexamethasone

FEBS Letters

Fisher, Sylvia C.¹  Brindley, David N.¹  Salter, Andrew M.¹ 

[1] Department of Biochemistry, University of Nottingham Medical School, Queens Medical Centre, Nottingham, NG7 2UH, England

关键词: Cholesterol metabolism;    Glucocorticoid;    Insulin;    LDL;    (Liver);   

DOI  :  10.1016/0014-5793(87)80895-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat hepatocytes were maintained in monolayer culture for 20 h in the presence of 10% (v/v) new-born calf serum and then for a further 1–24 h in serum-free medium containing 2 g bovine serum albumin/l. The specific binding of human ¹²⁵-I-LDL to two distinct sites was then measured at 4°C. Binding to site 1 was displaced by dextran sulphate while that to site 2 was not. The presence of 1–100 nM insulin for 24 h in the second incubation significantly increased binding to site 1. Significant increases were also seen when cells were incubated with 10 nM insulin for 1 h. No significant effects of insulin on binding to site 2 were observed. In contrast, 10 nM—1 μM dexamethasone decreased binding to both sites. The effects of these hormones were mutually antagonistic.

【 授权许可】

Unknown