Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of phosphorylase kinase

FEBS Letters

da Cruz e Silva, Edgar F.¹  Cohen, Patricia T.W.¹ 

[1] Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, Scotland

关键词: Phosphroylase kinase;    cDNA sequence;    Calmodulin;    X-linkage;    Codon usage bias;   

DOI  :  10.1016/0014-5793(87)80871-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthetic oligonucleotides have been used to isolate a 1.85 kb clone containing the full length coding sequence for the catalytic subunit of rabbit skeletal muscle phosphorylase kinase from a cDNA library constructed in λgt10. Sequence analysis of the clone predicted an amino acid sequence in agreement with a published primary structure. Inspection of the codon usage revealed a strong preference for G or C nucleotides at the third codon position as found for several other skeletal muscle proteins. This cDNA clone should facilitate identification of functional domains, including the calmodulin-binding site, and investigation of the molecular basis of X-linked phosphorylase kinase deficiencies.

【 授权许可】

Unknown