Production of the constant domain of murine T‐cell receptor β‐chain in Escherichia coli

FEBS Letters

Kurosawa, Yoshikazu³  Itoh, Seiga²  Kuwana, Yoshihisa³  Nagase, Fumihiko¹  Nakashima, Izumi¹ 

[1] Department of Immunology, Nagoya University School of Medicine, Tsurumai, Showa-ku, Nagoya 466, Japan;Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., 3-3-6 Asahimachi, Machida, Tokyo 194, Japan;Institute for Comprehensive Medical Science, Fujita-Gakuen Health University, School of Medicine, Toyoake, Aichi 470-11, Japan

关键词: T-cell receptor;    Expression vector;    Structural analysis;    aa;    amino acid(s);    bp;    base pairs;    Ig;    immunoglobulin;    MHC;    major histocompatibility complex;    PAGE;    polyacrylamide gel electrophoresis;    TCR;    T-cell antigen receptor;   

DOI  :  10.1016/0014-5793(87)80253-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

T-cell antigen receptor is a heterodimer of disulfide-linked α- and β-chains. Although the essential features of T-cell receptor seem to be rather similar to those of immunoglobulin, the amount of T-cell receptor expressed on the surface of a T-cell is not large enough to be analyzed physico-chemically. In this study, the DNA fragment encoding 120 amino acids from the 116th to the 235th of the murine, β-chain which corresponds to the presumed constant domain was inserted into an expression vector in E. coli. A large amount of this 18 kDa protein was observed to be synthesized in E. coli, and might be a good source for the three dimensional analysis of the T-cell receptor molecule.

【 授权许可】

Unknown