Ethanol alters the adenosine receptor‐Ni‐mediated adenylate cyclase inhibitory response in rat brain cortex in vitro

FEBS Letters

Giudicelli, Y.¹  Bauché, F.¹  Nordmann, R.¹  Bourdeaux-Jaubert, A.M.¹ 

[1] Department of Biomedical Research on Alcoholism and Department of Biochemistry, Faculty of Medicine Paris-West, 45 rue des Saints-Pères, 75006 Paris, France

关键词: Synaptosome;    Adenylate cyclase;    Enzyme inhibition;    Adenosine;    Ethanol effect;   

DOI  :  10.1016/0014-5793(87)80239-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has been suggested that ethanol stimulates adenylate cyclase in vitro through an increased function of N_s, the activitory component of adenylate cyclase. Because of the interaction of N_s with N_i, the adenylate cyclase inhibitory component, we have studied the effect of ethanol (0.05–0.2 M) on N_i-mediated adenylate cyclase inhibition caused by the adenosine analog N ⁶-phenylisopropyladenosine (N⁶-PIA) in brain cortical membranes. Ethanol did not alter N⁶-PIA binding to the adenosine R_i-receptors, stimulated slightly basal adenylate cyclase activity but abolished adenylate cyclase inhibition due to N⁶-PIA, suggesting an effect of ethanol on the inhibitory coupling pathway. This was further supported by loss of the adenylate cyclase inhibitory response to GTP (10⁻⁵ M). It thus seems that, besides its effect on the N_s system, ethanol may also impair N_i-mediated adenylate cyclase responses in rat cerebral cortex.

【 授权许可】

Unknown