Glucose transport in human erythrocytes measured using 13C NMR spin transfer

FEBS Letters

Potts, Jennifer R.¹  Chapman, Bogdan E.¹  Kuchel, Philip W.¹ 

[1] Department of Biochemistry, University of Sydney, Sydney, NSW 2006, Australia

关键词: Glucose transport;    13C-NMR;    Spin transfer;    (Human erythrocyte);   

DOI  :  10.1016/0014-5793(87)81180-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We present the results of a new NMR-based procedure for measuring the fast transmembrane exchange of D-[1-¹³C]glucose in human erythrocytes. The method relies on different rates of exchange between the α- and β-anomers of glucose inside and outside the cells; the rate outside the cells is greatly increased by the addition of mutarotase to the suspension. Theory is developed to describe nuclear-spin transfer in the present system and is used to analyse the data to yield estimates of transmembrane-exchange rate constants and their statistical uncertainties. For a total glucose concentration of 25.5 mmol/1 at 40°C the first order efflux rate constants for the α- and β-anomers were 1.20±0.40 s⁻¹ and 0.71±0.30 s⁻¹, respectively.

【 授权许可】

Unknown