The photosystem of the green sulfur bacterium Chlorobium limicola contains two early electron acceptors similar to photosystem I

FEBS Letters

Nitschke, Wolfgang¹  Hauska, Günter¹  Lockau, Wolfgang¹  Feiler, Ute¹ 

[1] Universität Regensburg, Institut für Botanik, Universitätsstrasse 31, 8400 Regensburg, FRG

关键词: Bacterial photosynthesis;    Reaction center;    Phototrapping;    EPR;    (Green sulfur bacteria;    Chlorobium);    DPPH;    1;    1-diphenyl-2-dipicrylhydrazyl radical;    PMSF;    phenylmethylsulfonyl fluoride;    EPR;    electron paramagnetic resonance;    P840;    primary electron donor of the reaction center of Chlorobium limicola;    PS;    photosystem;    RC;    reaction center;   

DOI  :  10.1016/0014-5793(87)81062-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An asymmetric EPR-line with a g factor of 2.0045 was detected after phototrapping at 196 K in membranes and in isolated photoactive P840 reaction centers of Chlorobium limicola f. thiosulfatophilum. The spectra resemble those of the electron acceptor A⁻ ₁ of photosystem I in chloroplasts and cyanobacteria. At 229 K a symmetric signal at g=2.0033, comparable to the one of the early electron acceptor A⁻ ₀ is additionally phototrapped. In contrast to membranes, the reaction center preparation does not contain appreciable amounts of photoreducible FeS-centers.

【 授权许可】

Unknown