Picosecond fluorescence of reaction centres from Rhodospirillum rubrum

FEBS Letters

Freiberg, A.M.¹  Godik, V.I.²  Kharchenko, S.G.²  Borisov, A.Yu.²  Rebane, K.K.¹  Timpmann, K.E.¹ 

[1] Institute of Physics of the Estonian SSR Academy of Sciences, Tartu 202400, Estonian, USSR;A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Bacterial photosynthesis;    Picosecond fluorescence;    Reaction center;    Primary charge separation;   

DOI  :  10.1016/0014-5793(85)81052-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The fluorescence kinetics of isolated Rhodospirillum rubrum reaction centres was studied. In the picosecond time range the decay was well approximated by two exponential components. The short-lived component was observed only when P₈₇₀ was reduced and had a maximal amplitude at about 910 nm. Its lifetime was equal to 7 ± 2 ps both at room temperature and at 77 K. It is inferred that this fluorescence component is emitted by excited photochemically active P₈₇₀ before the formation of an intermediate radical-pair state. The second, long-lived component with a lifetime of about 100–200 ps was observed in a wider spectral range and had maxima at about 850 and 900 nm both under reducing and oxidizing conditions. The bulk of this luninescence seemed to be emitted either by photochemically inactive P₈₇₀ and/or by traces of antenna bacteriochlorophyll.

【 授权许可】

Unknown