Molecular cloning of the myelin specific enzyme 2′,3′‐cyclic‐nucleotide 3′‐phosphohydrolase

FEBS Letters

Thompson, R.J.¹  Vogel, Ursula S.¹ 

[1] Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QR, England

关键词: CNPase;    Myelin;    cDNA sequence;   

DOI  :  10.1016/0014-5793(87)81058-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe the isolation of cDNA clones for bovine brain 2′,3′-cyclic-nucleotide 3′-phosphohydrolase (CNPase, EC 3.1.4.37), the third most abundant protein in central nervous system myelin. The cDNA encodes the complete protein (400 amino acids) and hybridizes to a major size species of mRNA in bovine brain tissue, approx. 2.7 kb in size. CNPase mRNA levels do not appear to be affected in quaking dysmyelinating mutant mice. The sequence reveals probable sites for CNPase phosphorylation by cAMP-dependent protein kinase and a region of homology with haemocyanin.

【 授权许可】

Unknown