FEBS Letters | |
Modulation of stimulatory action of follicle stimulating hormone (FSH) and inhibitory action of epidermal growth factor (EGF) on aromatase activity in Sertoli cells by calcium | |
Mallea, L.E.2  Machado, A.J.2  Navaroli, F.2  Rommerts, F.F.G.1  | |
[1] Department of Biochemistry, Division of Chemical Endocrinology, Erasmus University Rotterdam, Medical Faculty, Rotterdam, The Netherlands;Instituto Nacional de Endocrinologia, Zapata y D, Vedado, Habana, Cuba | |
关键词: Aromatization; EGF; Ca2+; Phorbol ester; (Sertoli cell); | |
DOI : 10.1016/0014-5793(87)81035-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Aromatization of testosterone by cultured Sertoli cells isolated from immature rats was stimulated more than 7-fold by follicle stimulating hormone (FSH) or dcAMP. The effects of FSH and dcAMP could be partly inhibited by epidermal growth factor (EGF) in a dose-dependent manner (ID50 0.5 nM). The phorbol ester 4β-phorbol-12-myristate-13-acetate (PMA) could also inhibit aromatase activity in a fashion similar to EGF. When 3 mM EGTA was present in the culture medium, the inhibitory effect of EGF was abolished but the stimulatory effect of FSH or dcAMP was magnified. These results suggest that EGF exerts a negative control on aromatase via calcium and protein kinase C. The abolishment of the inhibitory effect of EGF and the enhancement of the stimulatory effect of FSH or dcAMP by a calcium deficiency may be an indication that growth factors produced by Sertoli cells negatively control FSH-induced responses in an autocrine fashion.
【 授权许可】
Unknown
【 预 览 】
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