Modulation of stimulatory action of follicle stimulating hormone (FSH) and inhibitory action of epidermal growth factor (EGF) on aromatase activity in Sertoli cells by calcium

FEBS Letters

Mallea, L.E.²  Machado, A.J.²  Navaroli, F.²  Rommerts, F.F.G.¹ 

[1] Department of Biochemistry, Division of Chemical Endocrinology, Erasmus University Rotterdam, Medical Faculty, Rotterdam, The Netherlands;Instituto Nacional de Endocrinologia, Zapata y D, Vedado, Habana, Cuba

关键词: Aromatization;    EGF;    Ca2+;    Phorbol ester;    (Sertoli cell);   

DOI  :  10.1016/0014-5793(87)81035-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Aromatization of testosterone by cultured Sertoli cells isolated from immature rats was stimulated more than 7-fold by follicle stimulating hormone (FSH) or dcAMP. The effects of FSH and dcAMP could be partly inhibited by epidermal growth factor (EGF) in a dose-dependent manner (ID₅₀ 0.5 nM). The phorbol ester 4β-phorbol-12-myristate-13-acetate (PMA) could also inhibit aromatase activity in a fashion similar to EGF. When 3 mM EGTA was present in the culture medium, the inhibitory effect of EGF was abolished but the stimulatory effect of FSH or dcAMP was magnified. These results suggest that EGF exerts a negative control on aromatase via calcium and protein kinase C. The abolishment of the inhibitory effect of EGF and the enhancement of the stimulatory effect of FSH or dcAMP by a calcium deficiency may be an indication that growth factors produced by Sertoli cells negatively control FSH-induced responses in an autocrine fashion.

【 授权许可】

Unknown