Identification of the active site of Citrobacter freundii β‐lactamase using dansyl‐penicillin

FEBS Letters

Yamaguchi, Akihito¹  Sawai, Tetsuo¹  Adachi, Hideki¹ 

[1] Division of Microbial Chemistry, Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Chiba 260, Japan

关键词: Active site;    Dansyl-penicillin;    βLactamase;    Amino acid sequence;    (C. freundii);   

DOI  :  10.1016/0014-5793(87)81031-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The active site sequence of a β-lactamase encoded by chromosomal gene(s) in Citrobacter freundii GN346 was determined using dansyl-penicillin as a fluorescent probe. The tryptic digest of the labelled enzyme gave a fluorescent peptide containing 22 amino acids. The sequence of this peptide was identical to the consensus sequence of class C β-lactamases, Gly-Ser-X-Ser-Lys. The residue labelled was the serine adjacent to the glycine. The active site sequence corresponded to positions 46–67 of the entire sequence of the Citrobacter freundii β-lactamase determined on the basis of the DNA sequence of the structural gene [(1986) Eur. J. Biochem. 156, 441–445]. The labelled serine corresponded to Ser-64.

【 授权许可】

Unknown