Amino acid sequence around the active site cysteine residue of calcium‐activated neutral protease (CANP)

FEBS Letters

Hayashi, Hiroaki²  Iwai, Koichi²  Hayashi, Tomoko²  Suzuki, Koichi¹ 

[1] Department of Biochemistry, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Institute of Endocrinology, Gunma University, Maebashi 371, Japan

关键词: Ca2+-Activated neutral protease;    Thiol protease;    Amino acid sequence;    Active site;    Carboxymethylation;   

DOI  :  10.1016/0014-5793(83)80483-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The active site of calcium-activated neutral protease (CANP) was specifically labeled with iodoacetic acid. The active site hepta peptide was isolated from carboxymethylated CANP after digestion with proteases and the sequence was determined. The sequence indicates that CANP is a thiol protease and its comparison with other thiol proteases is described.

【 授权许可】

Unknown