Evidence for a trimeric organization of the photosystem I complex from the thermophilic cyanobacterium Synechococcus sp.

FEBS Letters

Witt, I.³  Boekema, E.J.¹  Saenger, W.³  Dekker, J.P.²  Rögner, M.²  van Heel, M.G.¹  Witt, H.T.² 

[1] Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, 1000 Berlin 33, Germany;>Max-Volmer-Institut für Biophysikalische und Physikalische Chemie, Technische Universität Berlin, Strasse des 17. Juni 135, 1000 Berlin 12, Germany;Institut für Kristallographie, Freie Universität Berlin, Takustr. 6, 1000 Berlin 33, Germany

关键词: Photosystem I;    Electron microscopy;    Structure analysis;    (Synechococcus);    APC;    allophycocyanin;    β-DM;    β-dodecyl-D-maltoside;    Car;    carotenoid;    Chl;    chlorophyll;    HPLC;    high-performance liquid chromatography;    OGP;    n-octyl-β-D-glucopyranoside;    PS;    photosystem;    SB;    sulfobetain;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(87)80679-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A photosystem I (PS I) reaction center complex was isolated and purified from the cyanobacterium Synechococcus sp. The complex has a molecular mass of about 600 kDa and contains 120 Chl a molecules per photoactive Chl a ^I (P-700). Electron micrographs show that the PS I complex has the shape of a disk with a diameter of about 19 nm and a thickness of 6 nm. Computer analysis reveals that the complex is composed of three similar units.

【 授权许可】

Unknown