Fluorimetric pH measurement in whole cells of dark aerobic and anaerobic cyanobacteria

FEBS Letters

Hinterstoisser, Barbara¹  Peschek, Günter A.¹ 

[1] Biophysical Chemistry Group, Institute of Physical Chemistry, The University of Vienna, Währingerstraße 42, A-1090 Vienna, Austria

关键词: Respiration;    Plasma membrane;    Proton translocation;    Fluorescence quenching;    intracellular pH;    Cyanobacteria;    CM;    plasma membrane;    ICM;    thylakoid membrane;    CS;    cytosol;    IT;    intrathylakoid compartment;    FDA;    fluorescein diacetate;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    Taps;    tris(hydroxymethyl)methylaminopropanesulfonic acid;    Mes;    2-(N-morpholino)-ethanesulfonic acid;   

DOI  :  10.1016/0014-5793(87)80657-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

9-Aminoacridine and atebrin fluorescence quenching by dark aerobic and anaerobic suspensions of Anacystis nidulans, Plectonema boryanum and Gloeobacter violaceus was determined at external pH 7–9. Individual pH values in cytosol and thylakoid compartments were calculated from the simultaneously different intracellular enrichment factors of the monovalent and the divalent base. Concomitantly, at external pH 4–7 the cytosolic pH was measured with fluorescein diacetate which is taken up and decomposed in the cell by cytosolic hydrolases. The pH-dependent fluorescence of the free fluorescein, which remains trapped in the cell, monitors the cytosolic pH. The fact that the latter was higher in aerobic than in anaerobic cells, and insensitive to saturating concentrations of dicyclohexylcarbodiimide aerobically, was taken to support the concept of a proton-translocating respiratory chain in the plasma membrane.

【 授权许可】

Unknown