Proton translocation coupled to methanogenesis from methanol + hydrogen in Methanosarcina barkeri

FEBS Letters

Blaut, Michael¹  Müller, Volker¹  Gottschalk, Gerhard¹ 

[1] Institut für Mikrobiologie der Universität Göttingen, Grisebachstr. 8, D-3400 Göttingen, FRG

关键词: Methanogenesis;    Proton translocation;    (M. barkeri);   

DOI  :  10.1016/0014-5793(87)80112-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of methanol to resting cells of Methanosarcina barkeri incubated under an atmosphere of molecular hydrogen resulted in an acidification of the medium. This acidification was not observed when H₂ was replaced by N₂ or air, or when the uncoupler tetrachlorosalicylanilide was present. 2-Bromoethanesulfonate completely inhibited both methanogenesis and proton extrusion. N,N′-Dicyclohexylcarbodiimide, an inhibitor of the proton-translocating ATPase in M. barkeri, did not affect proton extrusion. Therefore, it could be concluded that proton translocation was coupled to the terminal methylcoenzyme M methylreductase reaction and that it was not due to an H⁺-translocating ATPase. A maximum value of 4 H⁺ translocated per CH₄ formed was calculated.

【 授权许可】

Unknown