Purification and properties of an N‐acetylgalactosamine specific lectin from the plant pathogenic fungus Rhizoctonia solani

FEBS Letters

Vranken, An M.¹  Allen, Anthony K.²  Peumans, Willy J.¹  Van Damme, Els J.M.¹ 

[1] Katholieke Universiteit Leuven, Laboratorium voor Plantenteelt, Fakulteit der Landbouwwetenschappen, Kardinaal Mercierlaan, 92, B-3030 Leuven, Belgium;Department of Biochemistry, Charing Cross and Westminster Medical School, Hammersmith, London W6 8RF, England

关键词: Lectin;    Purification;    N-Acetylgalactosamine;    Gum arabic;    (Rhizoctonia solani);    PBS;    phosphate-buffered saline;    RSA;    Rhizoctonia solani agglutinin;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(87)80758-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A lectin was isolated from Rhizoctonia solani mycelium by affinity chromatography on gum arabic-Sepharose. It is a dimeric protein composed of 2 identical subunits of 13 kDa with high contents of asparagine/ aspartic acid, valine, glycine, glutamine/glutamic acid and lysine. The R. solani agglutinin (RSA) exhibits specificity towards N-acetylgalactosamine, and preferentially agglutinates human type A over type B and type O erythrocytes.

【 授权许可】

Unknown