Chemical cross‐linking of ferredoxin to spinach thylakoids

FEBS Letters

Zanetti, Giuliana¹  Merati, Giuliana¹ 

[1] Dipartimento di Fisiologia e Biochimica Generali, Via Celoria 26, 20133 Milano, Italy

关键词: Ferredoxin;    Thylakoid;    Ferredoxin-NADP+ reductase;    Photosystem I;    Photosynthesis;    EDC;    N-ethyl-3-(3-dimethylaminopropyl)carbodiimide;    Fd;    ferredoxin;    FNR;    ferredoxin-NADP+ reductase;    cyt.;    cytochrome;   

DOI  :  10.1016/0014-5793(87)80109-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ferredoxin has been chemically cross-linked to thylakoids by using N-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. The membranes thus treated became able to photoreduce cytochrome c and to catalyze the NADPH-cytochrome c reductase reaction without adding exogenous ferredoxin. Preincubation of thylakoids with an antibody against ferredoxin-NADP⁺ reductase before carbodiimide treatment or removal of the reductase by mild trypsin treatment after the cross-linking reaction did not alter the cytochrome c photoreduction activity of the treated membranes. Two independent binding sites of ferredoxin to thylakoids are thus inferred: one site is shown to be the membrane-bound reductase, the second is suggested to be at the level of the photosystem I complex.

【 授权许可】

Unknown