Photoinhibition: Impairment of the primary charge separation between P‐680 and pheophytin in photosystem II of chloroplasts

FEBS Letters

Demeter, Sandor¹  Melis, Anastasios¹  Neale, Patrick J.¹ 

[1] Division of Molecular Plant Biology, 313 Hilgard Hall, University of California, Berkeley, CA 94720, USA

关键词: Photoinhibition;    Charge separation;    Pheophytin;    Electron transport;    (Spinach chloroplast;    Chlamydomonas reinhardtii);    Chl;    chlorophyll;    PS;    photosystem;    QA;    primary quinone acceptor of PS II;    Pheo;    pheophytin primary electron acceptor of PS II;    P-680;    photochemical reaction center of PS II;    C f;    flattening correction factor;   

DOI  :  10.1016/0014-5793(87)80090-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The question of the primary site of photoinhibition was investigated using the light-induced absorbance change of pheophytin a (at 685 nm) in spinach chloroplasts and Chlamydomonas reinhardtii cells. Photoinhibition of spinach thylakoid membranes resulted in a parallel decrease in the amplitude of the pheophytin a (ΔA ₆₈₅) and Q_A (ΔA ₃₂₀) photoreduction signals. In intact Chlamydomonas reinhardtii cells the pheophytin photoreduction and oxygen evolution activity exhibited a similar decrease during photoinhibition. A complete recovery of both activities was attained within 60 min incubation in normal growth conditions. It is concluded that the primary site of photoinhibition involves the components (P-680 and/or pheophytin) of the primary charge separation.

【 授权许可】

Unknown