A comparison by magnetic circular dichroism of compound X and compound II of horseradish peroxidase

FEBS Letters

Field, Robert A.¹  Gadsby, Paul M.A.¹  Greenwood, Colin²  Foote, Nicholas²  Thomson, Andrew J.¹ 

[1] School of Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, England;School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, England

关键词: Horseradish peroxidase;    Compound II;    Compound X;    Magnetic circular dichroism;    HRP;    horseradish peroxidase;    P.+;    the protoporphyrin IX radical cation;   

DOI  :  10.1016/0014-5793(87)80085-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The chlorite product of horseradish peroxidase, compound X, is shown by magnetic circular dichroism (MCD) spectroscopy in the temperature range 1.6–50 K to have a very similar haem structure to compound II under the same conditions (pH 10.7). Both are concluded to contain the Fe(IV)=0 group. The MCD spectrum also detects an unusual species, absorbing at wavelengths between 600 and 750 nm, that has magnetic properties different from those of the ferryl haem group. It is suggested that this is a species at the same oxidation level as ferryl haem but with the porphyrin ring having suffered a one-electron oxidation, i.e. [Fe(III) P^.+].

【 授权许可】

Unknown