Na+ transport by reconstituted Na+,K+‐ATPase in the presence of various nucleotides

FEBS Letters

Svinukhova, Irina A.¹  Boldyrev, Alexander A.¹ 

[1] Department of Biochemistry, Moscow State University, Moscow 119899, USSR

关键词: (Na+ + K+)-ATPase;    Reconstitution;    Na+ transport;    Substrate specificity;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;   

DOI  :  10.1016/0014-5793(87)80082-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ability of ATP, CTP, ITP, GTP, UTP and two synthetic ATP analogs to provide for ouabain-sensitive Na⁺ accumulation into proteoliposomes with a reconstituted Na⁺,K⁺-ATPase (ATP phosphohydrolase, EC 3.6.1.37) was investigated. A correlation between the proton-accepting properties of the nucleotides and their ability to provide for active transport was found. The proton-accepting properties of the substrate seem to be a necessary condition for the shift from the K-form to Na form of Na⁺,K⁺- ATPase — an immutable step in the active translocation of Na⁺ and K⁺ through the Na⁺ pump.

【 授权许可】

Unknown