FEBS Letters | |
Na+ transport by reconstituted Na+,K+‐ATPase in the presence of various nucleotides | |
Svinukhova, Irina A.1  Boldyrev, Alexander A.1  | |
[1] Department of Biochemistry, Moscow State University, Moscow 119899, USSR | |
关键词: (Na+ + K+)-ATPase; Reconstitution; Na+ transport; Substrate specificity; FCCP; carbonyl cyanide p-trifluoromethoxyphenylhydrazone; | |
DOI : 10.1016/0014-5793(87)80082-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The ability of ATP, CTP, ITP, GTP, UTP and two synthetic ATP analogs to provide for ouabain-sensitive Na+ accumulation into proteoliposomes with a reconstituted Na+,K+-ATPase (ATP phosphohydrolase, EC 3.6.1.37) was investigated. A correlation between the proton-accepting properties of the nucleotides and their ability to provide for active transport was found. The proton-accepting properties of the substrate seem to be a necessary condition for the shift from the K-form to Na form of Na+,K+- ATPase — an immutable step in the active translocation of Na+ and K+ through the Na+ pump.
【 授权许可】
Unknown
【 预 览 】
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