Functional properties of nicotinic acetylcholine receptor subunits expressed in various combinations

FEBS Letters

Mishina, Masayoshi¹  Konno, Takashi¹  Numa, Shosaku¹  Tanaka, Ken-ichi¹  Fukuda, Kazuhiko¹  Kurosaki, Tomohiro¹  Mori, Yasuo¹ 

[1] Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto 606, Japan

关键词: Nicotinic acetylcholine receptor;    cDNA expression;    α-Bungarotoxin binding;    Agonist binding;    Channel activity;    (Xenopus oocyte);    ACh;    acetylcholine;    AChR;    acetylcholine receptor;    α-BTX;    α-bungarotoxin;   

DOI  :  10.1016/0014-5793(87)80065-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The four kinds of subunits of the Torpedo californica nicotinic acetylcholine receptor have been produced in various combinations by injecting Xenopus oocytes with the corresponding subunit-specific mRNAs synthesized by transcription in vitro of the cloned cDNAs. Functional analysis suggests that association of the α-subunit with either the γ- or the δ-subunit is a prerequisite for generating the conformation necessary for agonist binding. The acetylcholine receptor devoid of either the β-, γ- or δ-subunit exhibits weak channel activity.

【 授权许可】

Unknown