Coupling of D2 and D3 dopamine receptors to G‐proteins

FEBS Letters

Strange, Philip G.¹  Castro, Sandra W.¹ 

[1] Biological Laboratory, The University, Canterbury, Kent, CT2 7NJ, UK

关键词: D2 dopamine receptor;    D3 dopamine receptor;    G-protein;    Agonist binding;   

DOI  :  10.1016/0014-5793(93)81168-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recombinant cell lines expressing D₂ and D₃ dopamine receptor isoforms have been used to study coupling of these receptors to G-proteins from the effects of GTP to reduce agonist binding affinities in agonist/[³H]spiperone competition experiments. D_2(short) expressed in Ltk⁻ cells, D_2(long) expressed in Ltk⁻ or CHO cells and D₃ expressed in CHO cells all showed coupling to the endogenous G-proteins of the cells. The detailed agonist binding characteristics indicated that there were differences in receptor/G-protein coupling for the same receptor (D_2(long) expressed in two cell types or for different receptors (D_2(long),D_2(short) expressed in the same cell type.

【 授权许可】

Unknown