How to determine the efficiency of intermediate transfer in an interacting enzyme system?

FEBS Letters

Tompa, Péter¹  Batke, József¹  Ovádi, Judit¹ 

[1] Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Budapest, Hungary

关键词: Enzyme interaction;    Intermediate transfer efficiency;    Channeling;   

DOI  :  10.1016/0014-5793(87)80063-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A kinetic method, based upon measuring the transient time of coupled reactions, is proposed for the determination of the intermediate channel efficiency in a system of functionally interacting enzymes. The procedure rests upon a novel description in which the transient time is expressed as a function of channel efficiency and lifetime of the intermediate molecules. By this approach the reduction of transient time can be explained even if no changes in the kinetic parameters of the individual reactions occur. For determining channel efficiency, a linearized form has been evaluated and applied to the analysis of the kinetics of the aspartate aminotransferase-glutamate dehydrogenase coupled reaction, for which the data were taken from the literature [(1982) Eur. J. Biochem. 121, 511–517].

【 授权许可】

Unknown