Homology between bacterial DNA and bovine mitochondrial DNA encoding cytochrome c oxidase subunit III

FEBS Letters

Leffak, Michael¹  Prochaska, Lawrence J.¹  Fink, Pamela S.¹  Whitford, Tracy¹ 

[1] Department of Biological Chemistry, School of Medicine and College of Science and Mathematics, Wright State University, Dayton, OH 45435, USA

关键词: Cytochrome-c oxidase;    DNA hybridization;    DNA;    DNA homology;    (Bovine heart;    Bacteria);   

DOI  :  10.1016/0014-5793(87)80016-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A segment of mitochondrial DNA encoding the bovine cytochrome c oxidase subunit III gene was isolated and inserted into an Escherichia coli plasmid vector. A 556 base pair fragment of the insert DNA representing about 70% of the 3′-end of the subunit III gene was used to search for homology with bacterial DNA from strains that contain heme aa ₃-type cytochrome c oxidases. Bacillus subtilis, Thermus thermophilus, and PS3 DNAs all showed strong hybridization to the probe, whereas Paracoccus denitrificans and Rhodopseudomonas sphaeroides DNAs showed only weak hybridization to the probe, even under low stringency conditions.

【 授权许可】

Unknown