Computer‐aided design and physiological testing of a luteinising hormone‐releasing hormone analogue for ‘adjuvant‐free’ immunocastration

FEBS Letters

Morrison, Christopher A.²  Ward, David J.¹  Robson, Barry¹  Fishleigh, Robert V.¹ 

[1] Epsitron Peptide and Protein Engineering Research Unit, Immunology Division, Department of Cell & Structural Biology, The University, Oxford Road, Manchester M13 9PT, England;Department of Biochemistry & Molecular Biology, The University, Oxford Road, Manchester M13 9PT, England

关键词: Luteinising hormone-releasing hormone;    Structure prediction;    Molecular design;    Immunological castration;   

DOI  :  10.1016/0014-5793(87)80014-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An analogue of LHRH containing an extension of Gly-Cys at the carboxyl-terminus has been designed to permit reproducible coupling to a suitably modified carrier via a thioether bond. Potential energy calculations indicated that this analogue adopted a conformation in solution virtually identical to the type II′ turn around Gly-6—Leu-7 predicted for native LHRH. Intradermal administration of a conjugate of this analogue with purified protein derivative of tuberculin to male rats previously primed with BCG vaccine rapidly led to complete testicular regression. This adjuvant-free immunisation protocol may represent an alternative to castration for the veterinary control of reproductive function.

【 授权许可】

Unknown