FEBS Letters | |
Computer‐aided design and physiological testing of a luteinising hormone‐releasing hormone analogue for ‘adjuvant‐free’ immunocastration | |
Morrison, Christopher A.2  Ward, David J.1  Robson, Barry1  Fishleigh, Robert V.1  | |
[1] Epsitron Peptide and Protein Engineering Research Unit, Immunology Division, Department of Cell & Structural Biology, The University, Oxford Road, Manchester M13 9PT, England;Department of Biochemistry & Molecular Biology, The University, Oxford Road, Manchester M13 9PT, England | |
关键词: Luteinising hormone-releasing hormone; Structure prediction; Molecular design; Immunological castration; | |
DOI : 10.1016/0014-5793(87)80014-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
An analogue of LHRH containing an extension of Gly-Cys at the carboxyl-terminus has been designed to permit reproducible coupling to a suitably modified carrier via a thioether bond. Potential energy calculations indicated that this analogue adopted a conformation in solution virtually identical to the type II′ turn around Gly-6—Leu-7 predicted for native LHRH. Intradermal administration of a conjugate of this analogue with purified protein derivative of tuberculin to male rats previously primed with BCG vaccine rapidly led to complete testicular regression. This adjuvant-free immunisation protocol may represent an alternative to castration for the veterinary control of reproductive function.
【 授权许可】
Unknown
【 预 览 】
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RO201912020289057ZK.pdf | 409KB | download |