The cytoskeletal protein vinculin is acylated by myristic acid

FEBS Letters

Kellie, S.¹  Wigglesworth, N.M.¹ 

[1] Imperial Cancer Research Fund Laboratories, St. Bartholomew's Hospital, Dominion House, Bartholomew Close, London EC1A 7BE, England

关键词: Cytoskeleton;    Vinculin;    Acylation;    Myristic acid;    CEF;    chick embryo fibroblasts;    RSV;    Rous sarcoma virus;    SDS-PAGE;    SDS-polyacrylamide electrophoresis;    RIPA;    radioimmune precipitation;    Mes;    2-(N-morpholino)ethanesulfonic acid;    PMSF;    phenylmethylsulfonyl fluoride;    DMEM;    Dulbecco's minimal essential medium;    CSK;    cytoskeleton;   

DOI  :  10.1016/0014-5793(87)81536-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In non-muscle cells the mechanism by which microfilament bundles interact with the plasma membrane is unclear. Vinculin, a 130 kDa protein found in adhesion plaques, has been postulated to have a role as a membrane anchor for microfilaments and we have investigated the biochemistry of this molecule in more detail. We report that a fraction of vinculin in chick embryo fibroblasts is acylated by myristic acid. This modification was present in both membrane-bound, cytoskeletal and cytosolic vinculin and thus did not determine preferential subcellular localisation. Myristic acid was also present in vinculin from cells transformed by Rous sarcoma virus.

【 授权许可】

Unknown