Imaging of cell membraneous and cytoskeletal structures with a scanning tunneling microscope

FEBS Letters

Ruppersberg, Johann Peter¹  Hörber, J.K.Heinrich¹  Gerber, Christoph¹  Binnig, Gert¹ 

[1] Max-Planck-Institut für Medizinische Forschung, Abteilung Zellphysiologie, Jahnstraße 29, 6900 Heidelberg, FRG

关键词: Scanning tunneling microscope;    Cell membrane;    Cytoskeleton;    Intermediate filament;    Vinculin;   

DOI  :  10.1016/0014-5793(89)81596-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The first observation of unstained cell membraneous structures by a scanning tunneling microscope is reported. An adhesive preparation method was used for imaging human medulloblastoma cells from the cell line TE 671 and oocytes from the clawed toad Xenopus laevis. The images show filaments, stacks of molecules and hilly structures. The possible identity of the filamentous structures is discussed, although the observed structures cannot yet be fully characterized. The work suggests possible future experiments on various biological structures in their natural environment.

【 授权许可】

Unknown