Separation of damage specific DNA endonuclease activities present in calf thymus

FEBS Letters

Male, Rune¹  Helland, Dag E.¹  Kleppe, Kjell¹ 

[1] Laboratory of Biotechnology and Department of Biochemistry, University of Bergen, Bergen, Norway

关键词: DNA damage Endonuclease;    Purification;    Enzyme characterization;    (Calf thymus);   

DOI  :  10.1016/0014-5793(87)81494-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A DNA endonuclease activity present in calf thymus specific for incision on DNA damaged by ultraviolet light, osmium tetroxide, potassium permanganate, hydrogen peroxide and acid has been purified from whole cell extracts. The enzymatic activity was heterogeneous both with regard to molecular mass and charge. The molecular mass of the enzyme varied from 25 to 35 kDa, but the different enzymatic species appeared to possess similar activities. The enzymes acted equally well on damage in supercoiled and relaxed forms of DNA. It further had a narrow optimum with regard to salt concentrations, the optimum activity being observed at a concentration of KCl from 40 to 65 mM.

【 授权许可】

Unknown