期刊论文详细信息
FEBS Letters | |
Synthesis of dinucleoside tetraphosphates by RNA polymerase B (II) from calf thymus | |
Hinrichsen, Angelika I.1  Ortner, Irmgard M.1  Hartmann, Guido R.1  | |
[1] Institut für Biochemie, Ludwig-Maximilians-Universität, Karlstraße 23, D-8000 München 2, FRG | |
关键词: RNA polymerase B; (Calf thymus); Abortive initiation; Dinucleoside tetraphosphate; α-Amanitin; Heparin; | |
DOI : 10.1016/0014-5793(85)80150-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Highly purified RNA polymerase B (II) from calf thymus catalyses the synthesis of dinucleoside tetraphosphates from ribonucleoside triphosphates in the absence of an oligonucleotide primer or additional protein factors. The reaction requires a DNA template and bivalent cations such as Mn2+ or Mg2+. It is strongly inhibited by heparin and high concentrations of α-amanitin but not by rifampicin. On a given template various dinucleoside tetraphosphates of different sequence are formed although the yield depends on the nature of the template.
【 授权许可】
Unknown
【 预 览 】
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