Synthesis of dinucleoside tetraphosphates by RNA polymerase B (II) from calf thymus

FEBS Letters

Hinrichsen, Angelika I.¹  Ortner, Irmgard M.¹  Hartmann, Guido R.¹ 

[1] Institut für Biochemie, Ludwig-Maximilians-Universität, Karlstraße 23, D-8000 München 2, FRG

关键词: RNA polymerase B;    (Calf thymus);    Abortive initiation;    Dinucleoside tetraphosphate;    α-Amanitin;    Heparin;   

DOI  :  10.1016/0014-5793(85)80150-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Highly purified RNA polymerase B (II) from calf thymus catalyses the synthesis of dinucleoside tetraphosphates from ribonucleoside triphosphates in the absence of an oligonucleotide primer or additional protein factors. The reaction requires a DNA template and bivalent cations such as Mn²⁺ or Mg²⁺. It is strongly inhibited by heparin and high concentrations of α-amanitin but not by rifampicin. On a given template various dinucleoside tetraphosphates of different sequence are formed although the yield depends on the nature of the template.

【 授权许可】

Unknown