Affinity labelling of neuronal acetylcholine receptors localizes acetylcholine‐binding sites to their β‐subunits

FEBS Letters

Whiting, Paul¹  Lindstrom, Jon¹ 

[1] Receptor Biology Laboratory, The Salk Institute for Biological Studies, PO Box 85800, San Diego, CA 92138, USA

关键词: Acetylcholine receptor;    Nicotine;    Affinity labeling;    Monoclonal antibody;    (Brain);   

DOI  :  10.1016/0014-5793(87)81464-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neuronal nicotinic acetylcholine receptors (AChRs) from brains of chickens and rats consist of two types of subunits, α and β, of which a shares some antigenic determinants with α-subunits from AChRs of electric organ and muscle [(1986) Biochemistry 25, 2082-2093; (1986) J. Neurosci. G, 3061-3069; (1986) Proc. Natl. Acad. Sci. USA, in press]. Here we demonstrate that after reduction with dithiothreitol (DTT) the AChRs can be specifically labelled with the acetylcholine-binding site directed reagent 4-(N-maleimido)benzyltri [³H]methylammonium iodide. Labelling of the β-subunits of neuronal nicotinic AChRs indicates that the acetylcholine-binding site, and amino acids which may be homologous to Cys 192–193 of the α-subunits of AChRs from electric organ and muscle, are located on the β-subunit of neuronal AChRs. These results suggest that although neuronal nicotinic AChRs have some structural homologies to AChRs from muscle and electric organs, the AChRs from these sources are quite distant relatives in an extended gene family.

【 授权许可】

Unknown