Guinea pig neurohypophysial hormones

FEBS Letters

Rouillé, Y.¹  Acher, R.¹  Chauvet, M.T.¹  Chauvet, J.¹ 

[1]Laboratory of Biological Chemistry, University of Paris VI, 96, Boulevard Raspail, 75006 Paris, France

关键词: Oxytocin;    Arginine vasopressin;    Vasopressin-neurophysin precursor;    Neurohypophysial peptide;    Copeptin;    Processing enzyme;   

DOI  :  10.1016/0014-5793(87)81294-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Guinea pig neurohypophysial hormones have been purified by two procedures, one involving molecular sieving and paper chromatoelectrophoresis, the other high-pressure reverse-phase liquid chromatography. Arginine vasopressin and oxytocin have been identified by their amino acid compositions and their retention times in HPLC determined through their biological properties. No partially processed precursor, including a neurohormone and a neurophysin, has been detected. Because the cleavage of the three-domain vasopressin-neurophysin-copeptin precursor is apparently complete between the first two domains, whereas it is not between the second and the third, it is supposed that two distinct enzymic systems are involved in the processing.

【 授权许可】

Unknown