Effect of hypoosmolality on the abundance, poly(A) tail length and axonal targeting of arginine vasopressin and oxytocin mRNAs in rat hypothalamic magnocellular neurons

FEBS Letters

Mohr, Evita¹  Svane, Pernille C.²  Thorn, Niels A.²  Richter, Dietmar¹ 

[1] Institut für Zellbiochemie und klinische Neurobiologie, Universität Hamburg, Universitätskrankenhaus Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany;Danish Biotechnology Centre for Signal Peptide Research, Department of Medical Physiology, University of Copenhagen, The Panum Institute Building 12.2., Blegdamsvej 3, DK-2200 Copenhagen N, Denmark

关键词: Arginine vasopressin;    Oxytocin;    Messenger RNA;    Hypoosmolality;    Poly(A) tail size;    Axonal mRNAs;   

DOI  :  10.1016/0014-5793(95)01008-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Arginine vasopressin (AVP) and oxytocin (OT) mRNAs are targeted to the axonal compartment of rat hypothalamic magnocellular neurons. Salt-loading results in a considerable rise in hypothalamic and axonal AVP mRNA but only a moderate increase for axonal OT mRNA. Here we report that hypoosmolality gives rise to a rapid decrease of axonal AVP encoding transcripts to undetectable levels after 2 weeks. The levels of OT mRNA in the axonal compartment did not change significantly. In the hypothalamus the mRNA for AVP also decreased. The size of the poly(A) tract of AVP encoding transcripts appeared to be strictly correlated with plasma osmolality. In contrast, the amount and size of OT encoding mRNAs were only moderately or not influenced by hypoosmolar stimuli.

【 授权许可】

Unknown