Preliminary X‐ray studies of the tetra‐heme cytochrome c 3 and the octa‐heme cytochrome c 3 from Desulfovibrio gigas

FEBS Letters

LeGall, J.¹  Jensen, L.H.²  Sieker, L.C.² 

[1] Department of Biochemistry, School of Chemical Sciences, University of Georgia, Athens, GA 30602, USA;Department of Biological Structure. SM020, University of Washington, Seattle, WA 98195 USA

关键词: Multiheme cytochrome;    Cytochrome;    Low-potential cytochrome;    Crystallography;    Electron transport- (Desulfovibrio);   

DOI  :  10.1016/0014-5793(86)81124-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A tetra-heme and an octa-heme cytochrome c ₃ from the sulfate bacterium Desulfovibrio gigas have been crystallized. Diffraction quality crystals of the tetra-heme cytochrome are obtained from solution by the addition of polyethylene glycol at pH 6.5. The crystals are orthorhombic, space group P2₁2₁2 with unit cell parameters a = 42.27 Å, b = 52.54 Å and c = 52.83 Å. The octa-heme cytochrome crystals develop from low ionic strength solutions of phosphate or Tris-Cl in the pH range 6.2–7.6. The crystals belong to the trigonal system, space group P3₁ or the enantiomorph P3₂, with unit cell parameters a = b = 57.4 Å, c = 97.3 Å, γ = 120°. Single crystal diffraction studies of the structures of these two low-potential cytochromes are in progress.

【 授权许可】

Unknown